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Manipulation of environmental stress towards lutein production in chlorella fusca cell culture

Othman, Rashidi and Mohd Hassan, Norazian and Abu Bakar, Ainaa Eliah and Noh, Nur Hidayah and Ahmad Fadzlillah, Nurrulhidayah and Mahmad, Noraini (2019) Manipulation of environmental stress towards lutein production in chlorella fusca cell culture. Journal of Pharmacy and Nutrition Sciences, 9 (5). pp. 251-257. ISSN 2223-3806 E-ISSN 1927-5951

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Abstract

All carotenoids originate from a single, common precursor, phytoene. The colour of carotenoids is determined by desaturation, isomerization, cyclization, hydroxylation and epoxidation of the 40-carbon phytoene. The conjugated double-bond structure and nature of end ring groups confer on the carotenoids properties such as colour and antioxidant activity. Algae may become major sources of carotenoids but the extent of environmental stress and genetic influences on algae carotenoid biosynthesis are poorly understood. Carotenoid biosynthesis can be influenced by many aspects and is liable to geometric isomerization with the existence of oxygen, light and heat which affect the colour degradation and oxidation. Therefore, in this study carotenoid biogenesis is investigated in cell culture of Chlorella fusca as a potential model system for rapid initiation, and extraction of carotenoids by providing stringent control of genetic, developmental and environmental factors. The value of this experimental system for investigating key factors controlling the carotenoid accumulation is then tested by assessing the effects of environmental variables, such as drought stress, light intensity, nutrient strength and media formulation on carotenoid accumulation. Our findings revealed that the conversion of violaxanthin to lutein is due to irradiance stress condition, nutrient strength as well as drought stress. As a result, manipulation of environmental variables will up-regulate lutein concentration. This reaction will restrict the supply of precursors for ABA biosynthesis and the algae cell culture responds by increasing carotenogenic metabolic flux to compensate for this restriction. In conclusion, selecting the appropriate algae species for the appropriate environmental conditions is not only important for yield production, but also for nutritional value quality of carotenoid.

Item Type: Article (Journal)
Additional Information: 3842/75077
Uncontrolled Keywords: Carotenoid, phytoene, carotenogenesis, Chlorella fusca, violaxanthin, lutein
Subjects: Q Science > QD Chemistry
R Medicine > RS Pharmacy and materia medica
S Agriculture > SH Aquaculture. Fisheries. Angling > SH389 Fisheries-Algae and Algae Culture
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Architecture and Environmental Design > Department of Landscape Architecture
Kulliyyah of Architecture and Environmental Design
Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr rashidi othman
Date Deposited: 09 Oct 2019 11:03
Last Modified: 09 Oct 2019 11:03
URI: http://irep.iium.edu.my/id/eprint/75077

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