Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract

Lestari, Widya and Wan Yusry, Wan N A and Iskandar, Siti H. and Ichwan, Solachuddin J. A. and Irfan, Nining I. and Suriyah, Wastuti Hidayati (2019) Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract. Makara Journal of Health Research, 23 (2). pp. 121-126. ISSN 2356-3664 E-ISSN 2356-3656

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Abstract

Background: Myrmecodia pendans (M. pendans), or Sarang Semut, is an epiphyte with anticancer potential. It was recently reported that it induces apoptotic activity in the human oral squamous carcinoma (HSC-3) cell line. This study aimed to investigate the effect of M. pendans treated samples on the expression of apoptotic markers, Bax and Bcl-2. Methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was dried thoroughly and then extracted aqueously. The apoptotic activity was detected via flow cytometry. Bax and Bcl-2 expression was analyzed by quantitative real-time polymerase chain reaction. Results: Results of our cell cycle analysis reveal that aqueous extract of M. pendans induced apoptosis in 2.5 and 5 mg/mL but no change between these two concentrations. Apoptosis was observed at 24 h but not at 48 h. Bax and Bcl-2 expression in HSC-3 cells was affected by M. pendans. At 24 h, upregulation of Bax was observed at 2.5 mg/mL. However, after 48 h, Bax expression showed no changes at any concentration. Bcl-2 was significantly downregulated after 48 h of treatment. Conclusions: M. pendans extract induced apoptosis in HSC-3 cells, which might occur via the proapoptotic (Bax) and antiapoptotic (Bcl-2) pathways.

Item Type:	Article (Journal)
Additional Information:	6863/75057
Uncontrolled Keywords:	apoptosis, cell cycle, flow cytometry, real-time polymerase chain reaction
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences
Depositing User:	Dr Widya Lestari
Date Deposited:	30 Oct 2019 15:25
Last Modified:	07 Mar 2023 17:18
URI:	http://irep.iium.edu.my/id/eprint/75057

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