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Observation on antioxidant activity from leaves, callus and suspension culture of Justicia gendarusa

Amid, Azura and Johan, Nurul Najwa and Jamal, Parveen and Wan Mohd Zain, Wan Norzaidani (2011) Observation on antioxidant activity from leaves, callus and suspension culture of Justicia gendarusa. In: International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May, 2011 , Kuala Lumpur.

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Abstract

This Antioxidant activity and phenolic compound was found in Justicia gendarussa (JG) via total phenolic content (TPC) and α,α-diphenyl-β-pycrilhydrazil hydrate (DPPH) radical scavenging assays. The assays were applied on aqueous and methanolic extracts of leaves, callus culture and cell suspension culture of JG. Callus was induced from green and matured leaves of JG cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of 2,4- dichlorophenoxyacetic acid (2,4-D)or1-Naphthaleneacetic acid (NAA) and benzylaminopurine (BAP). Callus induced on NAA (1-Naphthaleneacetic acid) showed higher phenolic content and antioxidant activity compared to the one induced on 2,4-D. Highest total phenolic content; 88mg GAE/g was observed from callus culture induced with NAA and BAP while highest antioxidant activity (lowest IC50; 8.46x10-3mg/mL) was observed in the cell suspension

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 3688/7466
Uncontrolled Keywords: anti-oxidant; callus; Justicia gendarusa; suspension culture
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Azura Amid
Date Deposited: 29 Nov 2011 15:04
Last Modified: 16 Dec 2011 07:48
URI: http://irep.iium.edu.my/id/eprint/7466

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