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Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture

Chia, Ling Li and Jantan, Ibrahim and Chua, Kien Hui and Lam, Kok Wai and Rullah, Kamal and Mohd Aluwi, Mohd Fadhlizil Fasihi (2016) Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture. Frontiers in Pharmacology, 7. pp. 1-17. ISSN 1663-9812

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Abstract

Tocotrienols (T3) are well-known for their antioxidant properties besides showing therapeutic potential in clinical complications such as hyperlipidemia induced by diabetes. The aim of this study was to determine the effects of δ-T3, γ-T3, and α-T3 on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture. Pancreatic islets freshly isolated from male Wistar rats were treated with T3 for 1 h at 37°C in a microfluidic system with continuous operation. The cells were collected for total RNA extraction and reverse-transcribed, followed by measurement of insulin secretion-associated genes expression using quantitative real-time polymerase chain reaction. Molecular docking experiments were performed to gain insights on how the T3 bind to the receptors. Short-term exposure of δ- and γ-T3 to pancreatic β cells in a stimulant glucose condition (16.7 mM) significantly regulated preproinsulin mRNA levels and insulin gene transcription. In contrast, α-T3 possessed less ability in the activation of insulin synthesis level. Essentially, potassium chloride (KCl), a β cell membrane depolarising agent added into the treatment further enhanced the insulin production. δ- and γ-T3 revealed significantly higher quantitative expression in most of the insulin secretion-associated genes groups containing 16.7 mM glucose alone and 16.7 mM glucose with 30 mM KCl ranging from 600 to 1200 μM (p < 0.05). The findings suggest the potential of δ-T3 in regulating insulin synthesis and glucose-stimulated insulin secretion through triggering pathway especially in the presence of KCl.

Item Type: Article (Journal)
Additional Information: 9020/74082
Uncontrolled Keywords: Tocotrienols, insulin, microfluidic system, gene expression profiling, qRT-PCR, molecular docking
Subjects: R Medicine > RM Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr Kamal Rullah
Date Deposited: 18 Sep 2019 16:19
Last Modified: 18 Sep 2019 16:20
URI: http://irep.iium.edu.my/id/eprint/74082

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