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Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system

Zakaria, Zainul Amiruddin and Mahmood, Nor Diyana and Omar, Maizatul Hasyima and Bakhtiar, M. Taher and Basir, Rusliza (2019) Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system. Pharmaceutical Biology, 57 (1). pp. 335-344. ISSN 1388-0209 E-ISSN 1744-5116

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Abstract

Context: Muntingia calabura L. (Muntingiaceae) exerts antioxidant and anti-inflammatory activities, thus, it might be a good hepatoprotective agent. Objective: This study investigates the effect of methanol extract of M. calabura leaves (MMCL) on hepatic antioxidant and anti-inflammatory activities in CCl4-induced hepatotoxic rat. Materials and methods: Sprague Dawley rats (n 1⁄4 6) were treated (p.o.) with 10% DMSO (Groups 1 and 2), 50mg/kg N-acetylcysteine (Group 3) or, 50, 250, or 500mg/kg MMCL (Groups 4–6) for 7 consecutive days followed by pretreatment (i.p.) with vehicle (Group 1) or 50% CCl4 in olive oil (v/v) (Groups 2–6) on day 7th. Plasma liver enzymes and hepatic antioxidant enzymes and pro-inflammatory cytokines concen- trations were measured while liver histopathology was examined. Results: MMCL, at 500 mg/kg, significantly (p < 0.05) ameliorated CCl4-induced hepatotoxicity by decreas- ing the plasma level of alanine transaminase (429.1 versus 168.7U/L) and aspartate transaminase (513.8 versus 438.1 U/L) as well as the tissue level of nitric oxide (62.7 versus 24.1 nmol/g tissue). At 50, 250, or 500mg/kg, MMCL significantly (p<0.05) reduced the tumour necrosis factor a (87.8 versus 32.7pg/mg tissue), interleukin-1b (1474.4 versus 618.3pg/mg tissue), and interleukin-6 (136.7 versus 30.8pg/mg tis- sue) while increased the liver catalase (92.1 versus 114.4 U/g tissue) and superoxide dismutase (3.4 versus 5.5 U/g tissue). Additionally, qualitative phytochemicals analysis showed that MMCL contained gallic acid, ferulic acid, quercetin, and genistein. Discussion and conclusions: MMCL ability to attenuate CCl4-induced hepatotoxicity could be helpful in the development of hepatoprotective agents with fewer side effects.

Item Type: Article (Journal)
Additional Information: 5209/72035
Uncontrolled Keywords: Muntingiaceae; hepatoprotective activity; antioxidant activity; oxidative stress markers; pro-inflammatory mediators; phytoconstituents; UHPLC-ESI; GCMS
Subjects: R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr Muhammad Taher
Date Deposited: 09 May 2019 14:38
Last Modified: 12 Jul 2019 09:53
URI: http://irep.iium.edu.my/id/eprint/72035

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