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Non-alcoholic steatohepatitis animal model induction

Abdul Rahim, Roslina and Abdul Ghani, Azril Shahreez and Muhammad, Naznin and Abdullah, Nor Zamzila and Ramli, Norazsida (2019) Non-alcoholic steatohepatitis animal model induction. World Journal of Pharmacy and Pharmaceutical Sciences, 8 (4). pp. 1523-1538. ISSN 2278-4357

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Abstract

Aim: The aims of this study are to establish NASH animal model utilizing 1% and 12% cholesterol diet. Methods: This study begins with the induction phase with cholesterol diet. In the induction phase, 42 Sprague-Dawley rats were randomly divided into standard diet (std) group (n=12) and NASH group (n=30). The 12 rats in the std group were fed with commercial rat pellet while the 30 rats in the NASH group were fed with 1% cholesterol (1% CD) diet for 14 weeks. The experiment was extended for an additional 6 weeks with increment of cholesterol in the diet from 1% to 12 % in the NASH group since the liver histology did not show features of NASH in the 1% CD. Results: At the end of the additional 6 weeks period, liver histology showed NASH changes in the 12% CD. Results of the histological sections of the livers of the 12% CD rats in the induction phase exhibited features consistent with NASH which include macrovesicular steatosis, occasional hepatocyte ballooning degeneration with mild to moderate lobular and portal inflammation. The 12% CD group revealed significantly increased serum alanine aminotransferase (ALT), serum insulin levels, homeostatic model assessment insulin resistance (HOMA-IR), serum total cholesterol (TC), serum low-density lipoprotein cholesterol (LDL-c) and reduced high-density lipoprotein cholesterol (HDL-c) in rats. Conclusion: This study has successfully induced mild–moderate NASH rat model utilizing 12% cholesterol diet.

Item Type: Article (Journal)
Additional Information: 5456/71485
Uncontrolled Keywords: High cholesterol diet, Non-alcoholic steatohepatitis (NASH), liver histology, liver function test.
Subjects: Q Science > QP Physiology
R Medicine > R Medicine (General)
R Medicine > RB Pathology
R Medicine > RC Internal medicine
R Medicine > RC Internal medicine > RC803 Specialties of Internal Medicine-Diseases of The Digestive System. Gastroenterology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine > Department of Basic Medical
Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Pathology & Lab Medicine
Depositing User: Dr ROSLINA ABDUL RAHIM
Date Deposited: 05 Apr 2019 11:20
Last Modified: 05 Apr 2019 11:20
URI: http://irep.iium.edu.my/id/eprint/71485

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