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Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles

Khatun, Hanufa and Yusof, Faridah and Mohamed Azmin, Nor Fadhillah (2018) Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles. In: 5th International Conference on Biotechnology Engineering (ICBioE 2018), 19th-20th September 2018, Kuala Lumpur.

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Abstract

The purpose of this research is to optimize the immobilization of lipase from the viscera of channel catfish to magnetic nanoparticles (MNP-lipase) using CLEA technology, forming a new immobilized enzyme. Lipase was obtained from the viscera of channel catfish, using ammonium sulfate to salt it out. Magnetic nanoparticles were obtained using the co-precipitation method, in which ferric chloride (FeCl3.6H2O) and ferrous sulfate (FeSO4.7H2O) gave the Fe3+ and Fe2+ ions, and precipitation was done by adding ammonium hydroxide which enables the formation of iron oxide magnetic nanoparticles. The functionalized magnetic nanoparticles were immobilized to lipase using the CLEA method, and this immobilization process was optimized. Glutaraldehyde was used as the cross-linker, bovine serum albumin (BSA) was used as a proteic feeder, and acetone as the solvent. One Factor at a Time (OFAT) was used in the process of optimization. The significant factors were considered to be glutaraldehyde, acetone and BSA, and the optimum concentrations of these components were determined to be 80 mM, 60% v/v and 0.18 mM respectively, to produce an optimum new MNP-lipase from the viscera of channel catfish.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 4734/71470
Uncontrolled Keywords: magnetic nanoparticles, optimization, CLEA, enzyme immobilization, lipase, channel catfish
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Kulliyyah of Engineering
Depositing User: Dr Nor Fadhillah Mohamed Azmin
Date Deposited: 05 Apr 2019 14:54
Last Modified: 05 Apr 2019 14:54
URI: http://irep.iium.edu.my/id/eprint/71470

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