Culturing and maintaining mammalian cell culture

Ahmad Raus, Raha and Ali Salih, Ghassan and Shaban, Mustaffa Nameer (2018) Culturing and maintaining mammalian cell culture. In: Multifaceted protocol in biotechnology. Springer Nature, Singapore, pp. 215-226. ISBN 978-981-13-2256-3

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Abstract

Mammalian cell culture refers to culturing mammalian cells in a medium that provide nutrients for cells to be able to grow in vitro under environment that closely mimic the in vivo conditions. By enabling culturing these cells outside living biological entities, investigation on intra- and intercellular activities and flux; genetic and phenotyping analysis; proteomics, study of toxicology, drug discovery and development can be carried out without manipulation of living animals. In this chapter, detail protocol of media preparation, cell culture maintenance and preservation are elaborated for both types of mammalian cell culture, monolayer or suspension cultures. Determination of number of cells is discussed as well.

Item Type:	Book Chapter
Additional Information:	3911/70574
Uncontrolled Keywords:	mammalian cell culture
Subjects:	T Technology > TP Chemical technology > TP200 Manufacture and use of chemicals T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering
Depositing User:	Dr Raha Ahmad Raus
Date Deposited:	14 Feb 2019 11:27
Last Modified:	05 Mar 2020 15:51
URI:	http://irep.iium.edu.my/id/eprint/70574

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