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Monitoring the growth of plant cells in suspension culture

Mohamad Puad, Noor Illi and Abdullah, Tajul Afif (2018) Monitoring the growth of plant cells in suspension culture. In: Multifaceted protocol in biotechnology. Springer Nature, Singapore, pp. 203-214. ISBN 978-981-13-2256-3

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Abstract

Cell suspension cultures provide a convenient way in understanding the dynamics growth of plant cells. This knowledge is crucial in order to manipulate all parameters related to their growth so that the potential of plants can be fully utilised. Growth can be defined as the increase of cell numbers, size or mass. In a growth cycle, cells will be experiencing four phases which are lag, logarithmic (exponential), stationary and death phase. The information and knowledge gained from growth kinetics parameters provide the basic platform for modelling and predicting the characteristics of cell culture. This chapter describes the procedures for fresh weight, dry weight, TCN, medium conductivity (pH) and residual sugar analysis.

Item Type: Book Chapter
Additional Information: 5462/70386
Uncontrolled Keywords: Plant cell suspension · Cell growth · Fresh weight · Dry weight · Total cell number · Medium conductivity · Residual sugar analysis · Arabidopsis thaliana · Ficus deltoidea
Subjects: S Agriculture > SB Plant culture
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Kulliyyah of Engineering
Depositing User: Noor Illi Mohamad Puad
Date Deposited: 14 Feb 2019 10:24
Last Modified: 14 Feb 2019 10:24
URI: http://irep.iium.edu.my/id/eprint/70386

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