Site-directed mutagenesis on plasmid using polymerase chain reaction

Ahmad, Abdul Aziz and Mohd. Salleh, Hamzah and Noorbatcha, Ibrahim Ali (2018) Site-directed mutagenesis on plasmid using polymerase chain reaction. In: Multifaceted protocol in biotechnology. Springer Nature, Singapore, pp. 1-12. ISBN 978-981-13-2256-3

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Abstract

Site-directed mutagenesis (SDM) is a very useful technique to study changes in protein function that may occur as a result of the DNA manipulation. A detail procedure to employ SDM on whole plasmid using polymerase chain reaction (PCR) with successful application of the method on a pET28a plasmid harboring an endoglucanase I gene from Fusarium oxysporum is described here.

Item Type:	Book Chapter
Additional Information:	1813/70243
Uncontrolled Keywords:	Site directed mutagenesis · Endoglucanase · Fusarium oxysporum
Subjects:	T Technology > TP Chemical technology > TP200 Manufacture and use of chemicals T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Hamzah Mohd. Salleh
Date Deposited:	11 Feb 2019 15:23
Last Modified:	18 Jun 2021 10:30
URI:	http://irep.iium.edu.my/id/eprint/70243

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