Identification of VASA gene expression in in-vitro culture from non-obstructive azoospermia (NOA) testicular biopsy cells: A study to unlock knowledge of male infertility

Abdul Razak, Redzuan Nul Hakim and Md Isa, Muhammad Lokman and Muhammad Razali, Refaudeen and Mat Yusof, Afzan and Abdul Wahab, Azantee Yazmie and Ahmad Arifin, Syamsul and Ramli, Roszaman and Muhammad, Husin (2018) Identification of VASA gene expression in in-vitro culture from non-obstructive azoospermia (NOA) testicular biopsy cells: A study to unlock knowledge of male infertility. International Medical Journal Malaysia, 17 (Special Issue 2). pp. 143-148. ISSN 1823-4631

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Abstract

The expression product of VASA gene is widely conserved germ line marker and participates to regulate the development of reproductive system and spermatogenesis in healthy man. Azoospermic is a condition which the man is unable to produce any sperm cells for reproduction activity. This condition produced has bad impact to the man since the ability to produce their own progeny will be interrupted or blocked forever. Not only the family bloodline of the man would be ended, the psychological issue like shamefulness and low self-esteem occurs. In Islam, seeking knowledge is an obligatory to Muslims in order to solve problems and improve lives. Thus to unveil the problem of azoospermic man we aimed to determine the level of VASA gene expression in samples of testes cells of non-obstructive azoospermic (NOA) and compare it with sperms of healthy man. Samples were taken from three NOA patients by testicular sperm extraction (TESE) to obtain testicular biopsies. Testicular cells were isolated and cultured in supplemented knockout DMEM media. VASA gene expression was determined by reverse transcriptase polymerase chain reaction (RT-PCR). The VASA gene expression from sperm of healthy man was also determined for comparison purpose. It was demonstrated that the addition of growth factor significantly increased SSC-like cells colony formation in tissues obtained from NOA patients. No VASA expression was detected in spermatogonial-like stem cells culture on day 1, 7, 14 and 21 in each of the azoospermic samples. Our findings shown VASA gene was not expressed in spermatogenesis in vitro culture that might be associated with the abnormal differentiation of primordial germ cells that lead to male infertility. Islam also teaches us to not have feelings of despair and for problems that we are facing but to find the solution and consider the problem as a test from the Almighty. All the tests should be handled with strong believe since only Allah (SWT) knows what the best for us and each of the tests will have justified wisdom and benefits which we as human being are unable to realise thoroughly.

Item Type:	Article (Journal)
Additional Information:	5041/66348
Uncontrolled Keywords:	VASA, non-obstructive azoospermia, spermatogenesis, testicular cells, justified wisdom
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Obstetric & Gynecology Kulliyyah of Medicine > IIUM Fertility Centre Kulliyyah of Nursing > Department of Basic Medical Sciences for Nursing
Depositing User:	Dr Muhammad lokman M Isad
Date Deposited:	24 Sep 2018 12:14
Last Modified:	07 Aug 2020 08:27
URI:	http://irep.iium.edu.my/id/eprint/66348

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