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Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED)

Nordin, Nur Sazwi and Mokhtar@Makhtar, Khairani Idah and Md Isa, Muhannd Lokman and Syed Osman Idid, Syed Zahir Idid and Lestari, Widya and Mustafa Al-Ahmad, Basma Ezzat and Arief Ichwan, Solachuddin Jauhari and Darnis, Deny Susanti and Ahmad, Azlina and Kannan, Thirumulu Ponnuraj (2018) Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED). Journal of International Dental and Medical Research, 11 (2). pp. 643-649. ISSN 1309-100X E-ISSN 1309-100X

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Abstract

Previous study reported that plant natural product promotes stem cell growth. Flaxseed (Linum usitatissimum) contains numerous compounds recognized for its health benefits. This study aimed to investigate the biological effects of flaxseed crude extract on stem cells obtained from human exfoliated deciduous teeth (SHED). Whole flaxseeds were ground and extracted with absolute ethanol using soxhlet extractor. The effects of flaxseed crude extract on SHED were assessed at concentration 1, 2, 4, 8 and 16 mg/ml for cell viability using MTT assay, cell morphology using inverted microscope and proliferative activity describe as population doubling time (PDT) using alamarBlue assay. The fatty acid composition of flaxseed was analysed using gas chromatographymass spectrometry (GCMS) instrumental technique.Although insignificant, flaxseed at concentration up to 4 mg/ml slightly increased SHED proliferation activity while maintaining cell viability and morphology. However, 8 mg/ml of flaxseed inhibited cell viability and proliferation activity, and changed the cell morphology. GCMS analysis revealed the presence of linolenic acid, linoleic acid, palmitic acid and stearic acid. Overall, ethanolic crude extract of flaxseed at concentration up to 4 mg/ml slightly enhanced the growth and maintained the morphology of SHED.

Item Type: Article (Journal)
Additional Information: 7172/66347
Uncontrolled Keywords: Stem cell, flaxseed crude extract, cell viability
Subjects: R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Dentistry > Fundamental Dental and Medical Sciences
Depositing User: Assoc Prof Khairani Idah Mokhtar
Date Deposited: 02 Oct 2018 17:41
Last Modified: 08 Oct 2018 12:33
URI: http://irep.iium.edu.my/id/eprint/66347

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