Nordin, Nur Sazwi and Mokhtar@Makhtar, Khairani Idah and Md Isa, Muhannd Lokman and Syed Osman Idid, Syed Zahir Idid and Lestari, Widya and Mustafa Al-Ahmad, Basma Ezzat and Arief Ichwan, Solachuddin Jauhari and Darnis, Deny Susanti and Ahmad, Azlina and Kannan, Thirumulu Ponnuraj
(2018)
Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED).
Journal of International Dental and Medical Research, 11 (2).
pp. 643-649.
ISSN 1309-100X
E-ISSN 1309-100X
Abstract
Previous study reported that plant natural product promotes stem cell growth. Flaxseed (Linum
usitatissimum) contains numerous compounds recognized for its health benefits. This study aimed
to investigate the biological effects of flaxseed crude extract on stem cells obtained from human
exfoliated deciduous teeth (SHED). Whole flaxseeds were ground and extracted with absolute
ethanol using soxhlet extractor. The effects of flaxseed crude extract on SHED were assessed at
concentration 1, 2, 4, 8 and 16 mg/ml for cell viability using MTT assay, cell morphology using
inverted microscope and proliferative activity describe as population doubling time (PDT) using
alamarBlue assay. The fatty acid composition of flaxseed was analysed using gas chromatographymass
spectrometry (GCMS) instrumental technique.Although insignificant, flaxseed at
concentration up to 4 mg/ml slightly increased SHED proliferation activity while maintaining cell
viability and morphology. However, 8 mg/ml of flaxseed inhibited cell viability and proliferation
activity, and changed the cell morphology. GCMS analysis revealed the presence of linolenic acid,
linoleic acid, palmitic acid and stearic acid. Overall, ethanolic crude extract of flaxseed at
concentration up to 4 mg/ml slightly enhanced the growth and maintained the morphology of SHED.
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