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Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs

Mohamed, Nur-Aliana Hidayah and Mokhtar, Rafidah Hanim and Al-Ani, Imad Matloub Dally and Ayob, Azizi and Misran, Misni (2018) Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs. Makara Journal of Health Research, 22 (2). pp. 88-94. ISSN 2356-3664 E-ISSN 2356-3656

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Background: There is no histological study evaluating the effects of insulin-containing cream on skin injury. The goal of this study was to examine the effects of insulin-containing creams on wound healing. Methods: Creams consisting of nine parts of oil and one part of aqueous phase (9:1) mixed with 1.5 mL human insulin were prepared. Eighteen male guinea pigs were divided into three groups; the control (9:1 C) group received cream without insulin. The experimental groups received Humulin N (9:1 N) and Humulin R (9:1 R) respectively. A 1 cm2 wound of 1-2 cm thickness was created in the skin. Each animal received 0.5 g of the respective creams which was topically applied once a day for 14 days. The progress of wound healing was monitored daily. Skin tissues were excised at the 14th days from the wound sites and processed for light microscopy. Results: Skin wound treated with the long acting insulin Humulin N had an accelerated wound healing process with restoration of vascular network, increased collagen deposition and early complete wound remodeling. Conclusion: Insulin cream with long acting mechanism facilitates in normalizing cell permeability, promoting vascularization, reducing exudation and stimulate proliferation of cells. These properties render insulin cream suitable for expediting wound healing.

Item Type: Article (Journal)
Additional Information: 5849/66214
Uncontrolled Keywords: inflammation; Insulin cream; Guinea pigs; Skin; Wound healing
Subjects: Q Science > QM Human anatomy
Q Science > QP Physiology
R Medicine > RB Pathology
R Medicine > RL Dermatology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine > Department of Basic Medical
Kulliyyah of Medicine
Depositing User: Assoc. Prof Dr.. Imad Matloub Dally Al-Ani
Date Deposited: 02 Oct 2018 11:37
Last Modified: 31 Jan 2019 10:08
URI: http://irep.iium.edu.my/id/eprint/66214

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