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Optimum immobilization of β-Glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness

Maalim, Kauthar Yusuf and Jameel, Ahmed Tariq and Yusof, Faridah (2016) Optimum immobilization of β-Glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness. In: International Conference Biotechnology Engineering, ICBioE ‘16 July 25-27, 2016, Kuala Lumpur, Malaysia, 25-27 July 2016, Kuala Lumpur, Malaysia.

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Abstract

The optimum conditions for immobilization of β-Glucosidase on multiwalled carbon nanotubes (MWCNTs) and Ca-alginate beads were studied. The effectiveness of the reaction of the immobilized enzyme with pnitrophenyl-β-D-glucopyranoside as substrate is evaluated. For the same substrate concentration the activity of enzyme immobilized in Ca-alginate beads was lower than that of immobilization on MWCNTs. The optimum immobilization conditions were found to be 17 mg MWCNT and 4 hr incubation time for MWCNTs while for calcium-alginate gel, it was found to be 3.5 wt% Na-alginate and 2 hr incubation time. The immobilization yield of the enzyme on MWCNTs was found to be 63.91% and that of Ca-alginate beads was 53.56%. Upon subsequent use, the residual activity of the enzyme on MWCNTs was higher than that of the Ca-alginate beads, which lost almost 50% of the activity at the third run. Both Ca-alginate and MWCNT immobilized enzyme were found to follow Michaelis-Menten kinetics and were well represented by Langmuir plot which was finally used to determine Michaelis-Menten constants. The values of Vmax and Km for MWCNTs are 4.7 μmol/mL.min and 0.833 mM respectively while for Ca-alginate the constants are 4.428 μmol/mL.min and 0.2587 mM respectively.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 5642/63485
Uncontrolled Keywords: β-glucosidase enzyme; MWCNT; Ca-alginate; functionalization immobilization yield; residual activity; Micahelis-Menten kinetics; Langmuir plot
Subjects: T Technology > TP Chemical technology > TP155 Chemical engineering
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Ahmad Tariq Jameel
Date Deposited: 24 Apr 2018 10:12
Last Modified: 29 Jun 2018 11:25
URI: http://irep.iium.edu.my/id/eprint/63485

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