Fabrication of ceramic membrane chromatography for biologics purification

Mel, Maizirwan and Abdeen, Fouad and Sopyan, Iis (2009) Fabrication of ceramic membrane chromatography for biologics purification. In: Advanced materials and Processing Technologies, 26-29 October 2009, Kuala Lumpur.

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Abstract

Chromatography is one of the most important separation processes of choice for the recovery/purification of proteins and complex bio-structures. Fabrication of chromatographic membranes and their efficiency in the chromatography process has been the subject of many recent researches. In this study, a coin-like, 13 mm diameter and 3 mm thick, ceramic membrane was fabricated to be used as a chromatographic medium. The membrane is used to replace the conventional resin-based chromatography columns. Hydroxyapatite (HA) powder was used as a material for the membrane fabrication. In this project, a HA powder was produced using starch as pore creating agents. Characterization processes were done for the ceramic membrane using the suitable apparatuses. Three parameters of the fabrication process (starch wt %, compaction pressure and sintering temperature) were manipulated to optimize the performance of the membrane. The fabricated membrane was placed in a (FPLC) system to be tested for its performance as an adsorptive membrane. (IMAC) process was run by immobilizing Ni2+ ions at the membrane particles surfaces. NP protein of the (NDV) was used to test the membrane’s ability to bind Histidine-tagged proteins. The optimum set of process parameters that yielded in the highest porosity and good chromatogram was determined to be 5 wt % starch, 3000 psi compaction pressure and 1100°C sintering temperature.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	4085/6301
Uncontrolled Keywords:	Membrane chromatography, Porous ceramic membrane, IMAC, Hydroxyapatite, chromatography
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP200 Manufacture and use of chemicals T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering > Department of Manufacturing and Materials Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	24 Oct 2011 09:46
Last Modified:	22 Nov 2011 03:52
URI:	http://irep.iium.edu.my/id/eprint/6301

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