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Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)

Mel, Maizirwan and Arifin, Mohd Azmir and M Pauzi, Sazana (2009) Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF). In: 3rd International Conference on Chemical & Bioprocess Engineering , 12–14 August 2009, Kota Kinabalu, Sabah.

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Abstract

Currently, virus separation process is mainly conducted by using ultracentrifugation or sucrose gradient electrophoresis methods. Both methods however have several drawbacks wherein the ultracentrifugation method requires very high rotational speed to separate the virus while the sucrose gradient electrophoresis method is very time consuming. Alternative to both methods are by using Tangential Flow Filtration (TFF). In this study, we have separated and concentrated the Newcastle Disease Virus (NDV) harvested from embryonic specific pathogen free (SPF) eggs using TFF method by utilizing microfilter membrane with pore size of 0.45μm. Hollow fiber membrane was selected as filter because of their chemical and thermal stability. The study was conducted according to the design developed using Taguchi method which consists of selected three parameters and two levels of factors. The results revealed that higher pressure input (Pin), lower pressure output (Pout), and higher virus concentration led to higher virus titer. Optimum Trans-Membrane-Pressure (TMP) value of 15 psi and virus concentration of 28% had given the maximum titer of the virus which was 512 haemagglutination assay (HA) unit.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4085/6299 (Proceedings of the 3rd International Conference on Chemical & Bioprocess Engineering)
Uncontrolled Keywords: Separation, Tangential Flow Filtration (TFF), Newcastle Disease virus (NDV), hollow fiber membrane
Subjects: T Technology > TP Chemical technology > TP155 Chemical engineering
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Maizirwan Mel
Date Deposited: 24 Oct 2011 08:25
Last Modified: 22 Nov 2011 03:54
URI: http://irep.iium.edu.my/id/eprint/6299

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