Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors

Ibrahim, Irmanisha and Nordin, Anis Nurashikin and Mohd Mansor, Ahmad Fairuzabadi and Hashim, Yumi Zuhanis Has-Yun and Voiculescu, Loana (2017) Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors. Bulletin of Electrical Engineering and Informatics, 6 (4). pp. 317-326. ISSN 2089-3191

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Abstract

Monitoring the effectiveness of drugs on cancer cells is crucial for chemotherapeutics studies. Invitro cell-based biosensors can be used as an alternative for characteristic studies of cells’ response to drugs. Cell-based sensors provide real-time measurements and require smaller sample volumes compared to conventional T-flask measurement methods. This paper presents a biosensor that detects in real-time, impedance variations of human colon cancer, HCT-116 cells when treated with anti-cancer agent, 5-Fluorouracil (5-FU). Two different extracellular matrix (ECM); polyaniline and gelatin were tested and evaluated in terms of attachment quality. Polyaniline was found to provide the best attachment for HCT-116 cells and was used for cytotoxicity studies. Cytokinetic behavior indicated that 5-FU inhibited HCT-116 cells at IC50 of 6.8 µg/mL. Trypan blue exclusion method for testing cell viability was used to validate the impedance measurements, where the cancer cell concentrations were reduced to ~35% when treated with 2.5 µg/mL, and 50% when treated with 6.8 µg/mL. The results generated by the microfabricated impedance biosensor are comparable to the Trypan blue method since both gave similar cell growth trend. It can be concluded that the impedance biosensor has potential to be used as an alternative method in drug testing applications

Item Type:	Article (Journal)
Additional Information:	3239/62788
Uncontrolled Keywords:	Cell-based biosensor, Impedance spectroscopy, ECIS, Cancer, HCT-116, anti-cancer drug
Subjects:	Q Science > QR Microbiology T Technology > TA Engineering (General). Civil engineering (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering Kulliyyah of Engineering > Department of Electrical and Computer Engineering
Depositing User:	Dr. Anis Nurashikin Nordin
Date Deposited:	09 Apr 2018 11:15
Last Modified:	09 Apr 2018 11:15
URI:	http://irep.iium.edu.my/id/eprint/62788

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