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Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment

Ismail, Nurul Fitriatul Akmal and Sha'ban, Munirah and Noor Azmi, Azran Azhim (2017) Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment. In: 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2017), 11th-15th July 2017, Jeju Island, South Korea.

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Abstract

Aortic scaffolds prepared using sonication decellularization treatment has provided a successful medium for repopulation with vascular smooth muscle cells (VSMCs). The objective of this study is to explore the potential of tissue decellularization using ultrasonication treatment and its recellularization before implantation of the cell-seeded scaffolds into host. Aorta tissue samples are decellularized in 2% SDS with sonication for 10 hours and compared with the native tissues. The 4',6-diamidino-2-phenylindole (DAPI) staining was used to evaluate the decellularization and Hematoxylin-Eosin (H-E) staining was used to compare the VSMCs infiltrations onto the decellularized tissues at day-0 and day-6 after cell-seeding. The results histologically showed complete DNA removal from scaffolds after decellularization and subsequent recellularization resulted in successful VSMCs infiltration. Accordingly, the decellularized tissues treated with 2% SDS in sonication demonstrated successful VSMCs repopulation afterward and is speculated to have less toxicity and able to be effectively implanted into host.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 6006/61883
Uncontrolled Keywords: DNA, Electronic countermeasures, Acoustics, Scanning electron microscopy, Immune system, Structural rings
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Allied Health Sciences
Kulliyyah of Science > Department of Biotechnology
Depositing User: Ir Dr Azran Azhim Noor Azmi
Date Deposited: 06 Feb 2018 12:00
Last Modified: 26 Jun 2018 12:24
URI: http://irep.iium.edu.my/id/eprint/61883

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