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Trimmed off adipocytes: a source of newly secreted adipokines

Azahari, Nuraniza and Khattak, Muhammad Muzaffar Ali Khan and Mohd Shukri, Nor Azwani and Abdul Rahman, Suzanah and Syed Osman Idid, Syed Zahir Idid and Phang, Ing Chia and Md Isa, Muhammad Lokman and Md Aris, Mohd Aznan and Bakhtiar, M. Taher (2018) Trimmed off adipocytes: a source of newly secreted adipokines. Progress in Nutrition, xx (xx). pp. 1-6. (In Press)

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Abstract

Given the beneficial roles of adipokines on body metabolism and its profound protective effects against metabolic disease, a better understanding of the regulation of adipokines secretion is very important. The objective of this study was to isolate, detect and quantify the total proteins in trimmed off abdominal adipose tissues from halal meat sources namely chicken, beef and lamb. Abdominal adipose tissues were isolated from the aforementioned sources and delipidation of the tissues were performed by chloroform/methanol extractions. Then, the protein concentration was determined by using Protein Assay Bicinchoninate Kit method. This was followed by quantification of the adipokines using ELISA kit assay. The experiment was conducted in triplicates and the results are presented as means ± SD. Results indicate that the extraction of 10 gram subcutaneous adipose tissues from chicken, beef and lamb yielded 0.10 gram ofprotein amount which was 1.0 % from total tissue mass. The protein concentration in adipose tissue of chicken, beef and lamb were 1.25 ± 0.05, 1.75 ± 0.05 & 2.53 ± 0.07 mg/ml respectively. The isolated adiponectin concentration in chicken, beef and lamb were 158 ng/ml± 0.05, 24 ± 0.05µg/ml and 37 ± 0.08 ng/ml respectively. These sources will bear further investigation on isolation and formulation for diabetic rat models in near future. Keywords: Adipose tissue, protein, adiponectin

Item Type: Article (Journal)
Additional Information: 5145/61718
Uncontrolled Keywords: Adipose tissue, protein, adiponectin
Subjects: T Technology > TX Home economics > TX341 Nutrition. Foods and food supply
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences
Kulliyyah of Medicine > Department of Family Medicine (Effective: 1st January 2011)
Kulliyyah of Pharmacy
Kulliyyah of Science > Department of Biotechnology
Depositing User: Dr Muhammad Muzaffar Ali Khan Khattak
Date Deposited: 29 Mar 2018 11:27
Last Modified: 29 Mar 2018 11:27
URI: http://irep.iium.edu.my/id/eprint/61718

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