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Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells

Jaswir, Irwandi and Ahmad, Hasna and Darnis, Deny Susanti and Bakhtiar, M. Taher and Octavianti, Fitri (2017) Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells. International Food Research Journal, 24 (Suppl.). pp. 395-401. ISSN 1985-4668

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Abstract

In recent years, the search for natural, safe and effective therapies for the management of obesity has become important. The present study investigated the potential of brown seaweeds Sargassum oligocystum and Padina australis from Malaysian waters as natural alternatives for the management of obesity. Both seaweeds were macerated using acetone at room temperature for ten hours. The S. oligocystum extracts (SE) and P. australis extracts (PE) were then applied to 3T3-L1 cells during the differentiation stage and during the mature stage of the adipocyte life cycle to assess the effects of extracts on adipogenesis and adipolysis. Application of SE at 12.5 and 50 µg/ml to the cells decreased adipogenesis by 71.7%, and 84.8%, respectively, while cells treated with 12.5 and 50 µg/ml PE showed 85.7%, and 89.0% adipogenesis respectively, compared to control. Application of SE and PE to mature lipid cells stimulated adipolysis and the release of glycerol into the culture media. Application of SE at 12.5 and 50 µg µg/ ml in the cell media induced glycerol release by up to 88.6 and 93.0%, respectively, while PE increased glycerol release up to 92.9 and 95.6% respectively, compared to isoproterenol. This study demonstrates the potential of whole brown seaweed extracts from S. oligocystum and P. australis collected from Malaysian waters as natural anti- obesity agents. Incorporation of the brown seaweed into the diet as a functional food will be useful for prevention and treatment of obesity.

Item Type: Article (Journal)
Additional Information: 5784/60854
Uncontrolled Keywords: Adipogenesis, Adipolysis, Brown seaweeds, Extraction
Subjects: Q Science > QD Chemistry
T Technology > TP Chemical technology
Kulliyyahs/Centres/Divisions/Institutes: International Institute for Halal Research and Training (INHART)
Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr Irwandi Jaswir
Date Deposited: 13 Mar 2018 08:53
Last Modified: 19 Apr 2018 13:39
URI: http://irep.iium.edu.my/id/eprint/60854

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