The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed

Abdullah Sani, Muhamad Shirwan and Bakar, Jamilah and Abdul Rahman, Russly and Abas, Faridah (2017) The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed. International Food Research Journal, 24 (2). pp. 810-818. E-ISSN 2231-7546

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Abstract

Ten solvents were used to extract phytochemicals from the peel of Carica papaya cv. Sekaki/ Hong Kong to evaluate antibacterial activities and determine chemical composition of Carica papaya cv. Sekaki/Hong Kong seeds. The antibacterial activities of ten solvent extracts were tested against 14 microorganisms vis Shigella sonnei, Salmonella typhimurium, Escherichia coli, Salmonella enteritidis, Vibrio vulnificus, Vibrio parahaemolyticus, Proteus mirabilis, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Corynebacterium diphtheria, Clostridium perfringens, Streptococcus pneumoniae and Bacillus subtilis using disk diffusion test (DDT). The Carica papaya seed of ACN and MeOH extracts inhibited 11 microorganisms, indicating their broad spectrum activity. The disk diffusion test exhibited moderate and clear inhibition on C. diphtheria, S. pneumonia, B. subtilis and C. perfringens. MeOH extract inhibited S. enteritidis, V. vulnificus, P. mirabilis and B. cereus with the lowest MIC at 11.25 mg/mL each, thus was chosen as the best extract. The hierarchy of extract potency can be ranked as MeOH > acetone > ACN > CHCL3 > hexane > DE = PE > EtOH > DCM based on clear and moderate inhibition and the lowest MIC. TPC and TFC of the extracts ranged between 4.83 to 22.59 mg GAE/g DW and 1.32 (water) and 17.15 mg QE/g DW respectively. The GC/MS analysis of MeOH extract identified potential antibacterial compounds such as isothiocyanatomethyl benzene, 9-octadecenoic acid, hexadecanoic acid and β-sitosterol. The Carica papaya seed cv. Sekaki/ Hong Kong possessed significant antibacterial activities when extracted by different solvents in particular MeOH solvent.

Item Type:	Article (Journal)
Additional Information:	7834/59988
Uncontrolled Keywords:	Solvent extraction, Antibacterial properties, Papaya seed, Volatile components
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	Muhamad Shirwan Abdullah Sani
Date Deposited:	13 Dec 2017 10:50
Last Modified:	11 Jan 2018 10:19
URI:	http://irep.iium.edu.my/id/eprint/59988

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