A kinetic performance of Cross-Linked Enzyme Aggregates (CLEA)-amylase from Zophobas morio

Easa, Muhammad Noor and Yusof, Faridah and Abd Halim, Amanatuzzakiah (2017) A kinetic performance of Cross-Linked Enzyme Aggregates (CLEA)-amylase from Zophobas morio. International Food Research Journal (IFRJ), 24 (Suppl.). S320-S324. E-ISSN 2231-7546

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Abstract

Many studies have been done on various species of insects to investigate their potential use in industries. This is because insects have high protein content which could be further manipulated. Due to its eating habit, Zophobas morio larvae, also known as super mealworm has been shown to have high amylase activity. In this study, amylase from super mealworm has been immobilized via Cross-Linked Enzyme Aggregates (CLEA) technique and its kinetic performance, evaluated. CLEA is one of the best immobilization method with respect to enzyme stability and reusability. Kinetic performance of both free and CLEA-amylase were evaluated based on the Michaelis-Menten model. Results obtained based on Hanes-Woolf, Lineweaver-Burk, Eadie-Hofstee and Hyperbolic Regression plots showed that the kinetic parameters, Vmax and KM, changed upon immobilization. For CLEA-amylase, Hanes-Woolf plot showed the best-fitted model based on R2 with Vmax= 1.068 mM/min and KM= 0.182 mM, however, Lineweaver-Burk plot was used to obtain the kinetic parameters for free amylase, with Vmax and KM of 17.230 mM/min and 2.470 mM, respectively. Thus it is observed that upon immobilization, Vmax for amylase dropped appreciably, however, much lower substrate concentration is needed to saturate the enzymatic sites to reach its maximum catalytic efficiency. The result from this study might open the new path in discovering the potential use of insects in industrial applications, for example, making use of the recovered enzymes in the detergent industry.

Item Type:	Article (Journal)
Additional Information:	4734/59625
Uncontrolled Keywords:	Immobilization, Cross-Linked Enzyme Aggregates (CLEA), Kinetic Study, Michaelis-Menten Model, KM, Vmax
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	DR FARIDAH YUSOF
Date Deposited:	27 Nov 2017 17:29
Last Modified:	13 Mar 2018 14:46
URI:	http://irep.iium.edu.my/id/eprint/59625

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