Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture

Ahmad Nor, Yusilawati and Mel, Maizirwan and Mohd. Salleh, Hamzah and Ng, Kim Hooi and Wong, C. S. (2010) Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture. Medical Journal of Malaysia, 65. pp. 26-27. ISSN 0300-5283

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Abstract

The aim of this study is to develop a polymeric microcarrier (MC) for obtaining a higher cell density of Vero cells in spinner vessel. Different types of gelatin; bovine (BG), fish (FG), cationized bovine (CBG) and cationized fish (CFG) were covalently immobilized on carboxyl group containing polystyrene (COOH-PS). Their performance was evaluated by the cell loading test in Vero cell culture as carrier system. It was found that Vero cell has better performance on BG with highest maximum cell number at 1.45x105 cell/ml with good microscopic and suspension ability; easy sampling and easy cell recovery.

Item Type:	Article (Journal)
Additional Information:	4085/5950
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	29 Nov 2011 13:54
Last Modified:	06 Jul 2021 09:53
URI:	http://irep.iium.edu.my/id/eprint/5950

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