Optimization of fermentation media for citric acid production using empty fruit bunches as substrate by central composite design

Alam, Md. Zahangir and Jamal, Parveen and Basri, S Maryam H (2007) Optimization of fermentation media for citric acid production using empty fruit bunches as substrate by central composite design. In: International Conference on Biotechnology Engineering (ICBioE '07), 8-10 May, 2007, Kuala Lumpur. (Unpublished)

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Abstract

A solid state fermentation method will be carried out for the production of citric acid from empty fruit bunches (EFB) by employing the potential fungus Aspergillus niger (A106). Statistical optimization was carried out in terms of media composition and fermentation time by central composite design (CCD) to evaluate the polynomial regression model through effects of linear, quadratic and interaction of the factors. The factors involved were co-substrates concentration (glucose and wheat flour),nutrient concentration and fermentation day. The result from regression model and statistical analysis showed that glucose was the significant factor that contributed to the highest citric acid production. The maximum citric acid (3.9 g/l) was observed with the optimum fermentation conditions of 3% of glucose, 1% of wheat flour and 6% nutrient concentration, and 7 days of fermentation period.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	4157/5860
Uncontrolled Keywords:	Empty fruit bunches (EFB), citric acid, solid state bioconversion, statistical optimization, central composite design
Subjects:	T Technology > TP Chemical technology T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	31 Oct 2011 16:27
Last Modified:	22 Nov 2011 06:41
URI:	http://irep.iium.edu.my/id/eprint/5860

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