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Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients

Mehde, Atheer Awad and Mehdi, Wesen Adel and Yusof, Faridah and Ahmad Raus, Raha and Zainal Abidin, Zaima Azira and Ghazali, Hamid and Abd Rahman, Azlina (2017) Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients. International Journal of Biological Macromolecules, 105 (Part 1). pp. 1324-1327. ISSN 0141-8130

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Abstract

Background The intron 5 insertion/deletion polymorphism of Alpha-2-macroglobulin receptor-associated protein gene (Alpha-2-MRAP) has been implicated in numerous diseases. The current study was designed to analyze the association of intron 5 insertion/deletion polymorphism of Alpha-2-MRAP with nephrolithiasis patients. Methods PCR was conducted on genomic DNA of patients and control to look for Alpha-2-MRAP insertion/deletion polymorphism. Besides that, serum level of Alpha-2-MRAP, oxidative stress marker myeloperoxidase, Malondialdehyde (MDA), Advanced oxidation protein products (AOPP), and uric acid were determined. Results The D and I allele frequencies were 57.50% and 42.50% in patients, 77.50% and 22.50% in control, individually. The result showed that II genotype was associated with nephrolithiasis patients group. A significant decrease was observed in serum Alpha-2-MRAP,myeloperoxidase and TAS,while TOS,OSI,MDA,AOPP and uric acid were substantially increased in II and ID when compared to DD genotype in patients with nephrolithiasis. Conclusion Our results demonstrate for the first time that patients with II genotype had an increased risk of stones. Also, the results demonstrate that I allele of the 5 insertion/deletion polymorphism in the Alpha-2-MRAP gene is related with an increase of oxidative stress in nephrolithiasis patients and may possibly impose a risk for cardiovascular diseases in patients with II genotype of Alpha-2-MRAP.

Item Type: Article (Journal)
Additional Information: 4734/58529
Uncontrolled Keywords: Nephrolithiasis, Alpha-2-MRAP, gene polymorphism,Total antioxidant status
Subjects: R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Kulliyyah of Science > Department of Biotechnology
Depositing User: DR FARIDAH YUSOF
Date Deposited: 26 Sep 2017 11:57
Last Modified: 19 Mar 2018 12:31
URI: http://irep.iium.edu.my/id/eprint/58529

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