Statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate

Alam, Md. Zahangir and Abdul Karim, Mohamed Ismail and Mespuan, M T (2006) Statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate. In: 20th Symposium of Malaysian Chemical Engineers (SOMChE 2006), 19-21 December 2006, Shah Alam.

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Abstract

A laboratory-scale lignin peroxidase (LiP) or ligninase production from palm oil mill effluent (POME) by Phanerochaete chrysosporium in liquid state bioconversion (LSB) was studied. The effect of the concentration of substrate and co-substrate, inoculum size, initial pH of the medium, and the agitation speed were observed for maximum production of LiP. The statistical optimization of the physicochemical conditions by polynomial regression model through the effect of linear, quadratic and polynomial interaction were carried out for the enzyme production. The experimental design and optimization was done by using the fractional factorial design formulated by a statistical optimization software MINITAB. The lignin peroxidase activity was observed periodically by the enzyme assaying and other responses such as COD, TSS, as well as reducing sugar determination. The results showed that temperature of 40°C, substrate concentration of 2%, co-substrate concentration of 2%, pH 6, inoculum size of 2%, and agitation of 250 rpm maximize the LiP production in liquid state bioconversion of POME.

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	4157/5851
Uncontrolled Keywords:	Lignin peroxidase (LiP), liquid-state bioconversion, POME, fractional factorial design
Subjects:	T Technology > TP Chemical technology T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	02 Nov 2011 11:53
Last Modified:	22 Nov 2011 06:45
URI:	http://irep.iium.edu.my/id/eprint/5851

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