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Statistical optimization of process conditions for direct bioconversion of sewage treatment plant sludge for bioethanol production

Alam, Md. Zahangir and Kabbashi, Nassereldeen Ahmed and Razak, A.A. (2006) Statistical optimization of process conditions for direct bioconversion of sewage treatment plant sludge for bioethanol production. In: 3rd Kuala Lumpur International Conference on Biomedical Engineering 2006, 11-14 December, 2006, Palace of the Golden Horses, Malaysia.

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The production of bioethanol was conducted by utilizing domestic wastewater sludge as major substrate with the aid of yeast, Saccharomyces cerevisiae using liquid state bioconversion method (submerged fermentation). The optimi-zation of process conditions such as temperature, initial pH, inoculum dosage and agitation was carried out by using the central composite design (CCD) formulated by a statistical optimization software MINITAB. Optimization of process conditions was done with different ranges of temperature, pH, inoculum sizes and agitations with fixed media compositions obtained from previous study. A polynomial regression model was developed to determine the optimum compositions. Sever-al techniques such ANOVA, t-test, p-values were observed to evaluate the model as well as the optimization process. The maximum ethanol production (9.1% v/v) was found while model equation predicted ethanol production with 11.9% v/v using the optimum conditions: temperature of 330C, pH of 7, agitation of 200 rpm and inoculum of 1%. The results indicat-ed that the temperature was highly significant (p<0.01) fol-lowed by the pH (p<0.01), inoculum (p<0.05) and agitation rate (p<0.05). The coefficient of determination (R2) was 90.1% which satisfied the adjustment of experimental data in the model.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4157/5840, ISBN: 978-3-540-68016-1, ISSN: 1680-0737, IFMBE Proceedings Vol 15
Uncontrolled Keywords: Bioethanol, liquid state bioconversion, sewage treatment plant sludge, central composite design, optimization
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Md. Zahangir Alam
Date Deposited: 05 Nov 2011 11:08
Last Modified: 08 Nov 2014 15:53
URI: http://irep.iium.edu.my/id/eprint/5840

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