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Flavonoids from Tetracera indica Merr. induce adipogenesis and exert glucose uptake activities in 3T3-L1 adipocyte cells

Mahmudul Hasan, Md Mahmudul and Ahmed, Qamar Uddin and Mat So'ad, Siti Zaiton and Latip, Jalifah and Bakhtiar, M. Taher and Tg Zakaria, Tg Muhammad Faris Syafiq and Sarian, Murni Nazira and Alhassan, Muhammad Alhassan and Zakaria, Zainul Amiruddin (2017) Flavonoids from Tetracera indica Merr. induce adipogenesis and exert glucose uptake activities in 3T3-L1 adipocyte cells. BMC Complimentary and Alternative Medicine, 17 (1). pp. 1-14. ISSN 1472-6882

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Abstract

Background: Tetracera indica Merr. (Family: Dilleniaceae), known to the Malay as ‘Mempelas paya’, is one of the medicinal plants used in the treatment of diabetes in Malaysia. However, no proper scientific study has been carried out to verify the traditional claim of T. indica as an antidiabetic agent. Hence, the aims of the present study were to determine the in vitro antidiabetic potential of the T.indica stems ethanol extract, subfractions and isolated compounds. Methods: The ethanol extract and its subfractions, and isolated compounds from T. indica stems were subjected to cytotoxicity test using MTT viability assay on 3T3-L1 pre-adipocytes. Then, the test groups were subjected to the in vitro antidiabetic investigation using 3T3-L1 pre-adipocytes and differentiated adipocytes to determine the insulinlike and insulin sensitizing activities. Rosiglitazone was used as a standard antidiabetic agent. All compounds were also subjected to fluorescence glucose (2-NBDG) uptake test on differentiated adipocytes. Test solutions were introduced to the cells in different safe concentrations as well as in different adipogenic cocktails, which were modified by the addition of compounds to be investigated and in the presence or absence of insulin. Isolation of bioactive compounds from the most effective subfraction (ethyl acetate) was performed through repeated silica gel and sephadex LH-20 column chromatographies and their structures were elucidated through 1H–and 13C–NMR spectroscopy. Results: Four monoflavonoids, namely, wogonin, norwogonin, quercetin and techtochrysin were isolated from the T. indica stems ethanol extract. Wogonin, norwogonin and techtochrysin induced significant (P < 0.05) adipogenesis like insulin and enhanced adipogenesis like rosiglitazone. Wogonin and norwogonin also exhibited significant (P < 0.05) glucose uptake activity. Conclusion: The present study demonstrated that the flavonoids isolated from the T. indica stems possess antidiabetic potential revealing insulin-like and insulin-sensitizing effects which were significant among the compounds. This also rationalizes the traditional use of T. indica in the management of diabetes in Malaysia.

Item Type: Article (Journal)
Additional Information: 4921/58260
Uncontrolled Keywords: Keywords: Tetracera indica Merr., Flavonoids, 3T3-L1 preadipocyte cells, Adipogenesis, 2-NBDG glucose uptake activity, Insulin like activity, Insulin sensitizing activity
Subjects: R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr Muhammad Taher
Date Deposited: 06 Sep 2017 12:07
Last Modified: 21 Mar 2018 09:56
URI: http://irep.iium.edu.my/id/eprint/58260

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