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Statistical screening of medium components affecting lipase production using palm oil mill effluent by Penicillium citrinum

Salihu, Aliyu and Alam, Md. Zahangir and Abdul Karim, Mohamed Ismail and Hamzah, Mohd. Salleh (2011) Statistical screening of medium components affecting lipase production using palm oil mill effluent by Penicillium citrinum. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur.

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Abstract

A number of medium components influencing lipase production by Penicillium citrinum (ATCC 42799) were studied using palm oil mill effluent as the basal medium. The medium components (peptone, yeast extract, malt extract, NH4Cl, NaNO3, KH2PO4, CaCl2, MgSO4, olive oil and tween-80) were analyzed in twelve experimental trials using Plackett–Burman design. The most significant components affecting lipase production were found to be tween-80, peptone, yeast extract, malt extract and NaNO3 at p < 0.05. The results indicated the efficiency of using PB design for screening processes. However, optimal concentration of the significant components can be determined by further statistical analysis.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4157/5687 (Proceeding of the 2nd International Conference on Biotechnology Engineering (ICBioE 2011))
Uncontrolled Keywords: Lipase, Penicillium citrinum, Plackett–Burman design, palm oil mill effluent
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Md. Zahangir Alam
Date Deposited: 30 Oct 2011 23:13
Last Modified: 28 Dec 2012 09:00
URI: http://irep.iium.edu.my/id/eprint/5687

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