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Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices

Ali, Md Eaqub and Abdul Rashid, Nur Raifana and Abd Hamid, Sharifah Bee and Hossain, Shouquat M.Azad and ..,, Asing and Hossain, Md Amzad Motalib and Sarker, Md. Zaidul Islam (2017) Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices. International Journal of Food Properties, 20 (1). pp. 231-245. ISSN 1094-2912

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Abstract

The macaque (Macaca fascicularis) monkeys are the third largest primate population in tropical forests. Being the potential carrier of Simian Immunodeficiency and Ebola and Corona viruses, as well as being a religiously-protected and wildlife-protected species could not save the macaques from being over-hunted and consumed. Despite having needs, methods to detect monkey species in foods are rarely documented. To fill this gap, this article describes a monkey-specific polymerase chain reaction assay targeting a short-site (120 bp) of mitochondrial d-loop gene because the short-length targets are thermodynamically more stable than the longer ones under degrading states. Specificity was tested against 17 terrestrial and aquatic meat and fish species and no cross-species amplification was detected under raw, processed, and admixed states. The sensitivity of the assay was 0.0001 ng DNA under pure states and 0.1% monkey meats in binary meat mixtures. Finally, the assay was validated by digesting the polymerase chain reaction products with AluI and CViKI-1 and distinctive restriction fingerprints for macaque species were demonstrated. © 2017 Taylor & Francis Group, LLC.

Item Type: Article (Journal)
Additional Information: 6605/56745
Uncontrolled Keywords: Macaca fascicularis; Mitochondrial d-loop gene; Religious and wildlife restriction; Restriction fingerprints; Short-amplicon-length; PCR assay
Subjects: Q Science > QD Chemistry
Q Science > QP Physiology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Professor Dr. Md. Zaidul Islam Sarker
Date Deposited: 09 Jun 2017 04:20
Last Modified: 19 Jul 2017 07:08
URI: http://irep.iium.edu.my/id/eprint/56745

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