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Process optimization in citric acid production using sewage sludge as fermentation media

Mohamad Hanapi, Nur Hanis and Alam, Md. Zahangir and Abdulkarim, Mohd Ismail (2011) Process optimization in citric acid production using sewage sludge as fermentation media. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur.

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Abstract

A research has been conducted on liquid state bioconversion for the production of citric acid using Sewage Treatment Plant (STP) sludge as main substrate and added with sucrose as co-substrate. Three parameters were examined as to determine the best process optimization conducted in two liter (2L) bioreactor. Two level factorial designs with three parameters are applied for the fermentation. It was carried out using local isolates Aspergillus niger strain O103A at room temperature (30-32oC), agitation is set at three different values which are 100, 200 and 300rpm. Initial pH for fermentation is set at 5 and it was further adjusted to three levels which are 2, 3, and 4 on the second day of the fermentation. Another parameter is aeration where 1, 1.5 and 2 vvm were introduced. The fermentation was carried out for five days. The maximum yield of citric acid obtained throughout the fermentation is 23.68 g/L, which is due to low pH, low agitation and high aeration values.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4157/5652
Uncontrolled Keywords: Sewage Treatment Plant (STP) sludge, citric acid, Aspergillus niger, pH, aeration, agitation, two level factorial design
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Md. Zahangir Alam
Date Deposited: 30 Oct 2011 23:43
Last Modified: 22 Nov 2011 07:45
URI: http://irep.iium.edu.my/id/eprint/5652

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