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Purification and characterization of extracellular lipase from Candida cylindracea using palm oil mill effluent supplemented medium

Alam, Md. Zahangir and Salihu, Aliyu and Abdul Karim, Mohamed Ismail and Hamzah, Mohd. Salleh (2011) Purification and characterization of extracellular lipase from Candida cylindracea using palm oil mill effluent supplemented medium. In: International Conference of Chemical Engineering and Industrial Biotechnology (SOMChE-ICCEIB 2011), 28th Nov -1st Dec 2011, Teluk Cempedak, Kuantan. (In Press)

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Abstract

Production of Candida cylindracea lipase was optimized and highest lipase production was achieved after 36 hr of fermentation using palm oil mill effluent supplemented medium. The extracellular lipase was purified by DEAE-sepharose and Sephacryl S-200, exhibiting a single band with molecular mass of 58 kDa by SDS-PAGE and gel filtration. The temperature and pH optima of the enzyme were found to be 35oC and 8 respectively. The enzyme was stable at pH 7.0 and 8.0 for 12 hr and also at temperature range of 25 to 35oC for 1 hr. Among the divalent cation salts tested on the lipase activity, Ca2+ and Mn2+ activated the enzyme most, while Cu2+ and Fe2+ inhibited it. Non-ionic (Tween-80 and Tween-20 and Triton X-100) and ionic detergents (SDS) at 1% appeared to have stimulatory and inhibitory effects on lipase activity respectively. In case of stability in organic solvents, water miscible solvents are concentration dependent; where their low concentrations showed some stimulatory effects, with marked inhibition at higher concentrations. It can be concluded that knowledge of these properties of the enzyme as described in this study could go a long way in its effective utilization for industrial application processes.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4157/5629
Uncontrolled Keywords: Candida cylindracea, palm oil mill effluent, extracellular lipase, purification.
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Md. Zahangir Alam
Date Deposited: 01 Nov 2011 23:58
Last Modified: 22 Nov 2011 08:04
URI: http://irep.iium.edu.my/id/eprint/5629

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