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Separation and purification of cellulase from fermentation broth of sewage sludge

Mukhtaruddin, Muna Tasnim and Alam, Md. Zahangir and Mohd. Salleh, Hamzah and Rashid , Shah Samiur (2010) Separation and purification of cellulase from fermentation broth of sewage sludge. In: 2010 International Conference on Process Engineering and Advanced Materials (icpeam2010), 15th-17th June 2010, Kuala Lumpur Convention Centre, Kuala Lumpur.

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Abstract

In recent years, growing attention has been paid to the conversion of biomass into bioethanol which is considered the cleanest liquid fuel alternative to fossil fuels. One of the major applications of cellulase lies in the process of hydrolysis of cellulosic or lignocellulosic material to produce sugar which can be further fermented to produce ethanol. This study focuses on the purification of cellulases produced from fermentation broth of sewage sludge. In this study, crossflow filteration was used for the purification method using microfiltration (0.45 μm) and utrafiltration (10kD, 5 kD and 1 kD). The results revealed that ultrafiltration with membrane size of 1kD gave the best result for purification of cellulase from fermentation broth of sewage sludge which increases the enzyme activity from 22 U/ml after microfiltration to 50 U/ml after 1 kD of ultrafiltration.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4157/5585
Uncontrolled Keywords: cellulase
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Md. Zahangir Alam
Date Deposited: 01 Nov 2011 23:51
Last Modified: 25 Jun 2013 09:37
URI: http://irep.iium.edu.my/id/eprint/5585

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