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Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge

Drani , Afifah and Alam, Md. Zahangir and Muyibi, Suleyman Aremu (2009) Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge. In: Water Malaysia 2009 Conference & Exhibition, 19-21 May 2009, Kuala Lumpur. (Unpublished)

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Abstract

An optimization based on statistical design method was employed to optimize the media constituents for the cellulase production from sewage sludge through liquid state bioconversion (LSB) by filamentous fungus Trichoderma reesei RUT C-30. The statistical analysis of experimental result showed that the optimum media constituent for cellulase production using sewage sludge as the major substrate, containing 1.5% cellulose and 0.5% peptone, supplemented with 0.25% KH2PO4, 0.2% tween 80, and 0.03% MgSO4 gave the maximum cellulase activity of 21.33 U/ml (CMCase activity). Analysis of variance (ANOVA) showed a high coefficient of determination (R2) of 0.92 with very low probability value (p < 0.0001) for the developed model. The present model is a good prediction of the experimental results and it is highly significant.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4157/5576
Uncontrolled Keywords: Endo-β-1,4 glucanase activity (CMCase), Liquid State Bioconversion (LSB), Sewage Sludge, Trichoderma reesei
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Md. Zahangir Alam
Date Deposited: 03 Nov 2011 16:20
Last Modified: 22 Nov 2011 08:26
URI: http://irep.iium.edu.my/id/eprint/5576

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