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Optimization of the nutrient supplements for cellulase production with the basal medium palm oil mill effluent

Rashid, Shah Samiur and Alam, Md. Zahangir and Abdulkarim, Mohd Ismail and Salleh, Hamzah Mohd. (2009) Optimization of the nutrient supplements for cellulase production with the basal medium palm oil mill effluent. In: World Academic of Science, Engineering and Technology 2009, 25-27 December 2009, Bangkok, Thailand.

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Abstract

A statistical optimization was studied to design a media composition to produce optimum cellulolytic enzyme where palm oil mill effluent (POME) as a basal medium and filamentous fungus, Trichoderma reesei RUT-C30 were used in the liquid state bioconversion(LSB). 2% (w/v) total suspended solid, TSS, of the POME supplemented with 1% (w/v) cellulose, 0.5%(w/v) peptone and 0.02% (v/v) Tween 80 was estimated to produce the optimum CMCase activity of 18.53 U/ml through the statistical analysis followed by the faced centered central composite design(FCCCD). The probability values of cellulose (<0.0011) and peptone (0.0021) indicated the significant effect on the production of cellulase with the determination coefficient (R2) of 0.995.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4157/5574 ISSN 2010-376X (P)2010-3778 (O)
Uncontrolled Keywords: Face centered central composite design (FCCCD), Liquid state bioconversion (LSB), Palm oil mill effluent, Trichoderma reesei RUT C-30.
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Md. Zahangir Alam
Date Deposited: 03 Nov 2011 16:36
Last Modified: 03 Jan 2012 17:39
URI: http://irep.iium.edu.my/id/eprint/5574

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