Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests

Tukiran, Nur Azira and Ismail, Amin and Mustafa, Shuhaimi and Hamid, Muhajir (2015) Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests. Food Additives and Contaminants: Part A, 32 (7). pp. 1023-1028. ISSN 1944-0049 E-ISSN 1944-0057

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Abstract

Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 μg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng μg–1 (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry.

Item Type:	Article (Journal)
Additional Information:	7613/55414
Uncontrolled Keywords:	edible bird’s nest; gelatine; adulterant; ELISA
Subjects:	T Technology > TP Chemical technology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	Nur Azira Tukiran
Date Deposited:	23 Mar 2017 09:18
Last Modified:	18 Jul 2017 08:40
URI:	http://irep.iium.edu.my/id/eprint/55414

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