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Effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters

Abdullah, Nor Zamzila and A.Talib, Norlelawati and Ku Zaifah, Norsidah and Muhammad, Naznin and Mohamed, Abdul Hadi and Haderi, Fairuzzaidah (2016) Effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang.

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Abstract

Introduction: Preanalytical variation as a result of sample storage conditions are common issues in a large scale study since the sample has to be kept for an extended duration at ultra low temperature (-70ºC) before analysis. However, ultra low freezers may not be available in some laboratories. Sometimes, the laboratory test may need to be repeated on the previously frozen and thawed samples. The aim of this research was to study the effect of storage temperature and freeze-thaw cycles on some biochemical parameters in our laboratory. Methods: An experimental study was carried out on 50 volunteers. Serum were aliquoted and stored at 4ºC, -30ºC and -70ºC. The serum was analyzed for total cholesterol (TC), triglycerides (TG), high density-lipoprotein (HDL-C) and glucose after 3 months of storage. The effects of freeze-thaw cycles were also recorded. Results: No significant differences (p>0.05) were seen in the level of the biochemical parameters between the samples stored at -70ºC and at -30ºC. The levels of selected parameters were higher after second freeze-thaw cycles as compared to the first thaw. All parameters showed significant positive correlations between the samples stored at -70ºC and the samples stored at -30ºC and 4ºC as well as between the first and second freeze-thaw cycles in samples kept at both -70ºC and -30ºC. Conclusions: Our finding suggested that the level of TC, TG and glucose in sample stored for 3 months at -70ºC are comparable with the samples stored at -30ºC. Only one freeze-thaw cycle is acceptable.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 3746/55188
Uncontrolled Keywords: Serum storage, temperature, freeze-thaw cycle
Subjects: R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine > Department of Anaesthesiology & Intensive Care
Kulliyyah of Medicine > Department of Basic Medical
Depositing User: Dr Nor Zamzila Abdullah
Date Deposited: 27 Feb 2017 10:31
Last Modified: 27 Feb 2017 10:31
URI: http://irep.iium.edu.my/id/eprint/55188

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