Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell

Mel, Maizirwan and Mat Saad, Maizura and Hashim, Yumi Zuhanis Has-Yun and Mohamed Salleh, Moharnad Ramlan (2008) Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell. Asian Journal of Scientific Research, 1 (5). pp. 525-531. ISSN 1992-1454

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Abstract

Media optimization of RCI hybridoma cell culture for monoclonal antibody production was carried out in T -Flask experiment. The three identified important variables to affect the cell viability were studied. By using Central Composite Design of Response Surface Methodology(STATISTICA v 6.1) has shown that cell viability was mainly affected by glutamine, serum and NaC03 concentration, respectively. Among the 16 nillS tested, Rllll 11 indicated the best viability of the cell (>80% for five days). The critical values were obtained at 13.5, 1.68 and 0.87% for serum, glutamine and NaC03, respectively. These data were very significant where the p-values obtained for glutamine, serum and NaCO, were 0.000069, 0.003968 and 0.342151 (R' ~ 0.95476 and R Adj. ~ 0.88691), respectively.

Item Type:	Article (Journal)
Additional Information:	4085/5476
Uncontrolled Keywords:	Monoclonal antibody, RC1 hybridoma cell, cell viability, media optimization, central composite design, response surface method
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	17 Nov 2011 16:27
Last Modified:	02 Aug 2017 16:59
URI:	http://irep.iium.edu.my/id/eprint/5476

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