Comparative structural analysis of fruit and stem bromelain from Ananas comosus

Ramli, Aizi Nor Mazila and Abdul Manas, Nor Hasmaliana and Abdul Hamid, Azzmer Azzar and Abd. Hamid, Hazrulrizawati and Md. Illias, Rosli (2018) Comparative structural analysis of fruit and stem bromelain from Ananas comosus. Food Chemistry, 566. pp. 183-191. ISSN 0308-8146

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Abstract

Cysteine proteases in pineapple (Ananas comosus) plants are phytotherapeutical agents that demonstrate anti-edematous, anti-inflammatory, anti-thrombotic and fibrinolytic activities. Bromelain has been identified as an active component and as a major protease of A. comosus. Bromelain has gained wide acceptance and compliance as a phytotherapeutical drug. The proteolytic fraction of pineapple stem is termed stem bromelain, while the one presents in the fruit is known as fruit bromelain. The amino acid sequence and domain analysis of the fruit and stem bromelains demonstrated several differences and similarities of these cysteine protease family members. In addition, analysis of the modelled fruit (BAA21848) and stem (CAA08861) bromelains revealed the presence of unique properties of the predicted structures. Sequence analysis and structural prediction of stem and fruit bromelains of A. comosus along with the comparison of both structures provides a new insight on their distinct properties for industrial application.

Item Type:	Article (Journal)
Additional Information:	7089/53935
Uncontrolled Keywords:	Stem bromelain, Fruit bromelain, Amino acid analysis, 3D structure prediction
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology T Technology > TX Home economics > TX341 Nutrition. Foods and food supply
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology
Depositing User:	AP. Dr. Azzmer Azzar Abdul Hamid
Date Deposited:	25 Jun 2018 17:27
Last Modified:	18 Oct 2018 09:53
URI:	http://irep.iium.edu.my/id/eprint/53935

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