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Assessment of sulfide concentration effects in a microoxygenated condition on the growth and removal by pseudomonas putida (ATCC 49128)

Ahmad, Mani Malam and Azoddein, Abd.Aziz Mohd and Mohd Zahari, Mior Ahmad Khusairi and Abu Seman, Mazrul Nizam and Jami, Mohammed Saedi and Mohd Azli, Faten Ahada (2016) Assessment of sulfide concentration effects in a microoxygenated condition on the growth and removal by pseudomonas putida (ATCC 49128). Journal of Advanced Research in Materials Science, 27 (1). pp. 1-10. ISSN 2289-7992

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Micro-oxygenation has recently being gaining popularity as a successful biological sulfide oxidation technique from inflicted wastewater. In this study the application of orbital shaker as an alternative digester for sulfide bioxidation was experimented using Pseudomonas putida (ATCC 49128), under an oxygen tension or micro-oxygenated condition. Growth and sulfide reduction efficiency was measured spectrophotometrically under optimum physical conditions of pH, temperature, acclimatization time and agitation. Sulfide reduction was overwhelmingly recorded at three different sulfide loading rates of 200mM S2- L-1 d-1, 300mM S2- L-1 d-1 and 500mM S 2- L-1 d-1 with corresponding appreciable cell growth measured at OD600nm. The obtained results indicated that it was possible to realized sulfide removal efficiency of 96% to 100% within 24hrs, as well 45% to 70% within the first 6hrs of inoculation, with an overwhelming removal of 100% after 18hrs in 200mM and 300mM. On the other hand, the corresponding growth was 3.00 OD600nm, 2.824 OD600nm and 2.456 OD600nm in 500pmm, 200ppm and 300ppm concentration respectively. Based on this finding, it was clear that this inoculum can be employed to treat sulfide contaminated wastewater even at higher range under micro-oxygenated environmental condition.

Item Type: Article (Journal)
Additional Information: 5545/53780
Uncontrolled Keywords: Sulfide, Micro-oxygenated, Growth, Removal, Pseudomonas putida
Subjects: T Technology > TP Chemical technology > TP155 Chemical engineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Prof. Ir. Dr. Mohammed Saedi Jami, PhD CEng MIChemE
Date Deposited: 20 Dec 2016 11:47
Last Modified: 20 Dec 2016 11:47
URI: http://irep.iium.edu.my/id/eprint/53780

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