Targeting double genes in multiplex PCR for discriminating bovine,buffalo and porcine materials in food chain

Hossain, Mohammad Amzad Motalib and Ali, Md Eaqub and Abd Hamid, Sharifah EeBee Ee and ..,, Asing and Mustafa, Shuhaimi and Mohd Desa, Mohd Nasir and Sarker, Md. Zaidul Islam (2017) Targeting double genes in multiplex PCR for discriminating bovine,buffalo and porcine materials in food chain. Food Control, 73. pp. 175-184. ISSN 0956-7135

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Abstract

Beef, buffalo and pork are the major meat of economic, religious and health concern. Current methods to authenticate these materials in food chain are based on mainly single gene targets which are susceptible to break down by food processing treatments. We, for the first time, described here a double gene targeting short-amplicon length multiplex polymerase chain reaction assay for discriminating bovine, buffalo and porcine materials in a single assay platform. The advantage of the assay is evidenced in terms of fidelity, cost and time since it is highly unlikely that two different targets would be missing even in a decomposed specimen. Detection of multiple targets in a single assay definitely saves analytical cost and time. Mitochondrial cytochrome b (cytb) and ND5 genes were targeted and six different targets (length: 90–146 bp), two for each of cow (120 and 106bp), buffalo (90 and 138bp) and pig (73 and 146bp), were amplified from raw, boiled, autoclaved and microwaved cooked meat under pure and mixed matrices. The detection limit was 0.02 ng DNA under pure states and 0.1% meat in binary mixtures and meatball products. Screening of Malaysian meatball products revealed all beef products were buffalo positive in which 35% were totally replaced. In contrast, all pork products were found uncontaminated from beef and buffalo.

Item Type:	Article (Journal)
Additional Information:	6605/53774
Uncontrolled Keywords:	Decomposed specimens, double gene-targets, economic, meatball products, multiplex PCR, religious and health concern, short-length DNA targets
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Professor Md. Zaidul Islam Sarker
Date Deposited:	19 Jan 2017 15:18
Last Modified:	19 Jul 2017 08:40
URI:	http://irep.iium.edu.my/id/eprint/53774

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