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Optimisation of rhamnolipids produced by Pseudomonas aeruginosa 181 using Response Surface Modeling

Al-Araji, Laith Issa Yassin and Raja Abd Rahman, Raja Noor Zaliha and Basri, Mahiran and Salleh, Abu Bakar (2007) Optimisation of rhamnolipids produced by Pseudomonas aeruginosa 181 using Response Surface Modeling. Annals of Microbiology, 57 (4). pp. 571-575. ISSN 1590-4261

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Abstract

This work investigated the optimisation of the fermented culture medium for maximisation of rhamnolipids production produced by Pseudomonas aeruginosa 181 using Response Surface Modeling (RSM). A two full factorial central composite experimental design was used in the design of experiments and in the analysis of results. This procedure limited the number of actual experiments performed while allowing for possible interactions between the parameters (pH, stirring rate, casamino acid concentration and incubation period) on the production of biosurfactants. Production carried out at larger volumes of one litre using Bioreactor under RSM-optimised conditions yielded 3.61 g l−1 of products after purification by acid precipitation.

Item Type: Article (Journal)
Additional Information: 4999/5233
Uncontrolled Keywords: biosurfactant - rhamnolipid - Pseudomonas aeruginosa - production - RSM
Subjects: Q Science > QR Microbiology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Nursing
Depositing User: Assoc. Prof. Dr. Laith Issa Yassin
Date Deposited: 21 Nov 2011 08:49
Last Modified: 29 Nov 2011 12:09
URI: http://irep.iium.edu.my/id/eprint/5233

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