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Tualang honey improves non alcoholic steatohepatitis animal model

Abdul Ghani, Azril Shahreez and Abdullah, Nor Zamzila and Muhammad, Siti Aesah @ Naznin and Abdul Rahim, Roslina (2016) Tualang honey improves non alcoholic steatohepatitis animal model. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang, Malaysia. (Unpublished)

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Abstract

Introduction: Non-alcoholic Steatohepatitis (NASH) is an emerging chronic liver disease with limited therapy available. Studies utilizing animal models induced with cholesterol diet ranging from 1-2% are hampered by inconsistent yield of NASH features. Therefore, we aimed to establish a NASH animal model utilizing 12% cholesterol diet (CD) and to investigate the effects of Tualang honey (TH) known for its anti-inflammatory and anti-oxidative properties in this model. Methods: Twenty-four Sprague-Dawley rats were divided into 2 groups (12% CD and standard diet) and were fed for 6 weeks. Following the establishment of NASH, the rats in the 12% CD group were subsequently divided into 3 groups. The first group was continued with only 12% CD. In the other 2 groups in addition to the 12% CD they were given TH treatment at different concentrations (1.2 and 2.4 g/kg/day) for 4 weeks. Blood biochemical analysis and histological assessment of liver were subsequently performed. Results: The liver histological sections of the rats fed with 12% CD showed macrovesicular steatosis, ballooning degeneration with lobular and portal inflammation. They also had increased serum alanine aminotransferase (ALT), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), fasting insulin, HOMA-IR and reduced high density lipoprotein cholesterol (HDL-C). Meanwhile, the TH treatment groups exhibited significant improvement in both the NASH grading and activity scores. The ALT, LDL-C, TC, triglyceride (TG), fasting insulin and HOMA-IR levels were reduced significantly. Conclusions: The 12% CD was able to induce NASH in the animal model. Tualang honey improved insulin sensitivity, dyslipidaemia, steatohepatitis.

Item Type: Conference or Workshop Item (Poster)
Additional Information: 3746/51857
Uncontrolled Keywords: NASH, cholesterol diet, Tualang Honey
Subjects: Q Science > QP Physiology
R Medicine > R Medicine (General)
R Medicine > RA Public aspects of medicine
R Medicine > RB Pathology
R Medicine > RC Internal medicine
R Medicine > RD Surgery
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine > Department of Basic Medical
Kulliyyah of Medicine
Depositing User: Dr ROSLINA ABDUL RAHIM
Date Deposited: 09 Sep 2016 10:25
Last Modified: 09 Sep 2016 10:25
URI: http://irep.iium.edu.my/id/eprint/51857

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