Formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: Gross observation, histology and immunohistochemistry analysis

Sha'ban, Munirah and Mohamad Sukri, Norhamiza and Ahmad Radzi, Muhammad Aa’zamuddin and Abdul Rahman, Rozlin and Mohamad, Mohd Yusof and Md Nazir, Noorhidayah and Zulkifly, Ahmad Hafiz and Hashi, Abdurezak Abdulahi (2016) Formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: Gross observation, histology and immunohistochemistry analysis. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.

PDF - Published Version Restricted to Repository staff only Download (1MB) | Request a copy

Abstract

This study aimed to explore the formation of hyaline-like articular cartilage substitute using primary cells derived from osteoarthritic patients overexpressed with SRY(Sex Determining Region Y)-Box9 (SOX9)geneseeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin as cells carrier through in-vitro and in vivo experiment settings using the ectopic implantation model i.e. athymic mice. Based on the approved research protocol, the TECs were formed and cultured for three weeks in vitro. The TECs were then implanted subcutaneously at the dorsum of athymic mice for four weeks. All TECs were subjected to gross inspection, histology, and immunohistochemistry (IHC) evaluation. Macroscopically, all in vitro TECs exhibited smooth, white and glossy hyaline-like cartilage appearance. After implantation, the 2-week TECs showed a favourable hyaline-like cartilage appearance when compared to the 4-week TECs. Histologically, all in vitro TECs exhibited minimally or lack cartilaginous tissue formation. The presence of high density, lacunae-encapsulated chondrocytes, distributed in cartilaginous matrices was observed in most postimplanted TECs. Coupled with positive immunoreaction against collagen type II (the cartilage-specific marker), cartilaginous properties were noticeable more in the 2-week than the 4-week post-implanted TECs.

Item Type:	Conference or Workshop Item (Invited Papers)
Additional Information:	6006/51700
Uncontrolled Keywords:	articular cartilage; chondrocytes; osteoarthritis; SOX9 overexpression; gene transfer; PLGA; fibrin; tissue engineered construct; ectopic implantation model
Subjects:	R Medicine > RD Surgery > RD701 Orthopedics T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation Kulliyyah of Science > Department of Biotechnology
Depositing User:	Munirah Sha'ban
Date Deposited:	19 Aug 2016 11:22
Last Modified:	09 Dec 2022 11:45
URI:	http://irep.iium.edu.my/id/eprint/51700

Actions (login required)

View Item