Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy

Ben Belgacem, Farah Fadwa and Bellag, Oualid Abdel Kader and Noor Batcha, Ibrahim Ali and Mohd Salleh , Hamzah (2016) Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.

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Abstract

Green technology is the environment-friendly way to protect the Earth’s natural resources and the environment we inhabit. The functionalmetagenomic approach has proven to be a powerful tool to identify novel biocatalysts indispensables in green technology of biofuel production from plant’s biomass through metagenomic DNA library construction and high-throughput screening. Culture enrichment strategies are additional pre-screening methods to provide an attractive mean of enhancing the screening hit rate. In this work metagenomic DNA libraries were made from Malaysian palm oil mill effluent (POME) microorganisms after culture enrichment experiment based on natural selection principle. Metagenomic DNA was extracted from both enriched and non-enriched cultures and cloned to pCC1FOS fosmid and transformed into EPI300™-T1RE. coli.Qualitative and quantitative analysis of metagenomic DNA and recombinant clones are provided to confirm the efficiency of enrichment strategy.

Item Type:	Conference or Workshop Item (Invited Papers)
Additional Information:	3704/51691
Uncontrolled Keywords:	cellulose-degrading enzyme, Malaysian palm oil mill effluent (POME), enrichment, metagenomic DNA library, high-throughput screening (HTS).
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof. Dr. Ibrahim Ali Noorbatcha
Date Deposited:	19 Aug 2016 09:50
Last Modified:	19 Aug 2016 09:50
URI:	http://irep.iium.edu.my/id/eprint/51691

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