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Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake

Riyadi, Fatimah Azizah and Alam, Md. Zahangir and Salleh, Md Noor and Mohd Salleh, Hamzah (2016) Screening of thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.

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Special consideration has been focused on the utilization of lipase enzyme as a suitable alternative method for biodiesel production, which is expected to be more stable and practical. However, the high production costs as well as low productivity have hindered the marketable production of biodiesel using the commercial lipase. Isolation of lipases from filamentous fungi, particularly found in agricultural wastes has attracted special interest due to their potential towards developing more sustainable and economical applications. Only lipase possessing thermal stability and organic solvent tolerant can distinctively enhance the biodiesel production. Therefore, in the present study, isolation of thermostable- organic solvent tolerant fungal strains from palm kernel cake (PKC) were conducted based on their ability to grow at 45oC on YpSs (yeast extract + starch + agar) media and in the presence of organic solvent. The isolates were screened based on the clearing zone methods on phenol red as well as on tween 80 agar media. Four isolates were screened for potential lipase production. These isolates were incubated under Solid State Fermentation (SSF) at 45oC for 7 days with PKC as substrate. The most promising strain with the potential to produce lipase was PKC12B2, which showed satisfactory enzyme activity.

Item Type: Conference or Workshop Item (Invited Papers)
Additional Information: 4157/51681
Uncontrolled Keywords: Thermostable organic solvent-tolerant lipase, Filamentous fungi, Palm kernel cake, Solid state fermentation
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Md. Zahangir Alam
Date Deposited: 18 Aug 2016 14:03
Last Modified: 26 Feb 2018 16:12
URI: http://irep.iium.edu.my/id/eprint/51681

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