A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat

Al-Mahmood, Sinan and Abdul Razak, Tariq and Che Abdullah, Shahrin T and NA, Nik Nur Fatnoon and Mohamed, Abdul Hadi and Al-Ani, Imad Matloub Dally (2016) A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat. Makara Journal of Health Research, 20 (2). pp. 48-56. ISSN 2356-3664 E-ISSN 2356-3656

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Abstract

Objective: The purpose of this study was to provide a reference of chronic diabetes complications by investigating the prolonged hyperglycemia effects on hematological, biochemical and histopathological changes (liver, kidney, spleen, cardiac muscle, adrenal gland, and endocrine pancreas) in diabetic rats induced by streptozotocin. Methods: Ten adult female Sprague-Dawley of uniform age were divided into two Groups. Group 1 was made diabetic by single intraperitoneal injection of streptozotocin (60 mg/kg/bw) whereas Group 2 served as control. After six months, the rats were anesthetized using pentobarbital. Cardiac puncture was performed to get 3 ml of the blood sample; following 12 hours of an overnight fast. Serum chemistry test and complete blood analysis for lipid profile and blood glucose test; liver and renal functions were performed. Tissue specimens of liver, kidney, spleen, cardiac muscle, adrenal gland, and endocrine pancreas were fixed in 10% formal saline and processed for histological study. Results: There were severe histopathological changes in the affected organs; and the presence of a significant abnormality of lipid profile, liver, and renal functions. Conclusions: The presence of histopathological changes with abnormal biochemical changes is related to the chronic absence of insulin production in the destroyed β –cells which reflect the diabetic complications in a human being.

Item Type:	Article (Journal)
Additional Information:	7037/51478
Uncontrolled Keywords:	biochemical, histopathological, rats, streptozotocin
Subjects:	R Medicine > R Medicine (General) R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Nursing
Depositing User:	Mr SINAN AL-MAHMOOD
Date Deposited:	09 Aug 2016 15:20
Last Modified:	09 Oct 2017 09:23
URI:	http://irep.iium.edu.my/id/eprint/51478

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