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Improvement of production of citric acid from oil palm empty fruit bunches: Optimization of media by statistical experimental designs

Bari, Md. Niamul and Alam, Md. Zahangir and Muyibi, Suleyman Aremu and Jamal, Parveen and Al-Mamun, Abdullah (2009) Improvement of production of citric acid from oil palm empty fruit bunches: Optimization of media by statistical experimental designs. Bioresource Technology, 100 (12). pp. 3113-3120. ISSN 09608524

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Abstract

A sequential optimization based on statistical design and one-factor-at-a-time (OFAT) method was employed to optimize the media constituents for the improvement of citric acid production from oil palm empty fruit bunches (EFB) through solid state bioconversion using Aspergillus niger IBO-103MNB. The results obtained from the Plackett–Burman design indicated that the co-substrate (sucrose), stimulator (methanol) and minerals (Zn, Cu, Mn and Mg) were found to be the major factors for further optimization. Based on the OFAT method, the selected medium constituents and inoculum concentration were optimized by the central composite design (CCD) under the response surface methodology (RSM). The statistical analysis showed that the optimum media containing 6.4% (w/w) of sucrose, 9% (v/w) of minerals and 15.5% (v/w) of inoculum gave the maximum production of citric acid (337.94 g/kg of dry EFB). The analysis showed that sucrose (p < 0.0011) and mineral solution (p < 0.0061) were more significant compared to inoculum concentration (p < 0.0127) for the citric acid production.

Item Type: Article (Journal)
Additional Information: 4157/5059
Uncontrolled Keywords: Citric acid Oil palm empty fruit bunches (EFB) Optimization Solid state bioconversion Aspergillus niger
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Md. Zahangir Alam
Date Deposited: 04 Nov 2011 11:57
Last Modified: 21 Nov 2011 10:47
URI: http://irep.iium.edu.my/id/eprint/5059

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