Solid State bioconversion of oil palm biomass for ligninase enzyme production

Alam, Md. Zahangir and Mahmat, Mohd Erman and Muhammad, Nurdina (2005) Solid State bioconversion of oil palm biomass for ligninase enzyme production. Artificial Cells, Blood Substitutes, and Biotechnology, 33 (4). pp. 457-466. ISSN 1073-1199 print/1532-4184 online

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Abstract

A laboratory-scale study of bioconversion of local lignocellulosic material, oil palm biomass (OPB) was conducted by evaluating the enzyme production through microbial treatment in solid state bioconversion (SSB). OPB in the form of empty fruit bunches (EFB) was used as a solid substrate and treated with the white-rot fungus, Phanerochaete chrysosporium, to produce ligninase. The results showed that the highest ligninase activity of 400.27U=liter was obtained at day 12 of fermentation. While the optimum study indicated the enzyme production of 1472.8U=liter with moisture content of 50%, 578.7U=liter with 10%v=w of inoculum size, and 721.8U=liter with co-substrate concentration of 1%(w=w) at days 9, 9 and 12 of fungal treatment, respectively. The parameters glucosamine and reducing sugar were observed to evaluate the growth and substrate utilization in the experiment.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Ligninase (LiP); Solid state bioconversion; Oil palm biomass (OPB);Phanerochaete chrysosporium
Subjects:	T Technology > TP Chemical technology T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	16 Jul 2013 14:37
Last Modified:	16 Jul 2013 14:37
URI:	http://irep.iium.edu.my/id/eprint/5033

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